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Nanodisc

2022-5-23 13:46:24 人评论

Nanodiscs-Pic2.jpg

Description

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50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1D1deltaH5-His protein and DMPC + 10% biotinylated PE lipids. For use in cell-free reactions.

Product is shipped on dry ice - additional freight charges apply.

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产品简介:

Nanodisc可使膜蛋白处于一个类似磷脂双分子层的环境中,从而保证膜蛋白能够像在天然的细胞膜中维持其构象和生物学功能。Nanodisc这种全新的膜蛋白提取工具打破了原有提取方法的瓶颈,能够很好地维持膜蛋白的稳定,如此可以推动膜蛋白领域的研究工作。Nanodisc是由膜支架蛋白(membrane scaffold proteins,MSPs)和磷脂分子构成的磷脂双分子层类膜结构。整合到Naonodisc中的膜蛋白可以保持其生物学活性,为膜蛋白的研究提供了便利。

根据不同的实验情况,科研人员可以使用两种不同的策略来包裹其想要获得的膜蛋白。对于已经分离并溶解在去污剂中的膜蛋白,我们推荐使用膜支架蛋白(MSPS)并配合磷脂分子使用。针对不同的需求我们提供多种膜支架蛋白和多种磷脂分子以及不同配合的试剂盒供选择。而用于无细胞表达系统所生产的膜蛋白,我们推荐使用已经组装好的空载纳米磷脂盘来包裹/插入其目标膜蛋白。您可以直接购买组装好的纳米磷脂盘或相应的试剂盒自行组装。

产品优势:

1)德国制造, 欧美领先蛋白纯化产品供应商;

2)专用于膜蛋白的研究,提供带有His-tag和不带标签的MSPs膜支架蛋白(人源和鼠源);

3)提供四种不同尺寸的MSPs膜支架蛋白 (MSP1D1/MSP1D1-dH5/MSP1E3D1/MSP2N2);

4)分别提供配备DMPC、POPC和DMPG磷脂的试剂盒(根据研究发现,不同膜蛋白在不同磷脂双分子环境下其活性有所不同,例如:真核磷脂环境和原核磷脂等环境,可按照实际需求订购,见图1);

5)详尽的操作指南和专业技术支持;

6)配合PureCube Rho1D4 Agarose使用,是有效的膜蛋白提取解决方案;

7)Nanodisc系列产品MSP1D1-His和MSP1D1dH5-His的应用已刊登在《美国化学会-应用材料与界面》(ACS Applied Materials & Interfaces): Zeno, W.F. et al. Spectroscopic Characterization of Structural Changes in Membrane Scaffold Proteins Entrapped wit hin Mesoporous Silica Gel Monoliths. Applied Materials & Interfaces 2015, 7, 8640-8649.

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图. 1: E. coli MraY转位酶在不同磷脂环境中的稳定性和活性。 数据来源于法兰克福大学Frank Bernhard

What our customers say:

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订购信息:

26112

MSP1D1-His,   lyophilized protein (2 mg)

26116

MSP1D1-His,   lyophilized protein (10 mg)

26122

MSP1D1 dH5-His,   lyophilized protein (2 mg)

26126

MSP1D1 dH5-His,   lyophilized protein (10 mg)

26152

MSP1E3D1-His,   lyophilized protein (2 mg)

26156

MSP1E3D1-His,   lyophilized protein (10 mg)

26172

MSP2N2-His,   lyophilized protein (2 mg)

26176

MSP2N2-His,   lyophilized protein (10 mg)

26512

mouse MSP1D1-His,   lyophilized protein (2 mg)

26516

mouse MSP1D1-His,   lyophilized protein (10 mg)

26552

mouse   MSP1E3D1-His, lyophilized protein (2 mg)

26556

mouse   MSP1E3D1-His, lyophilized protein (10 mg)

26211

Nanodisc Assembly   Kit MSP1D1-His_DMPC

26213

Nanodisc Assembly   Kit MSP1D1-His_POPC

26221

Nanodisc Assembly   Kit MSP1D1 dH5-His_DMPC

26223

Nanodisc Assembly   Kit MSP1D1 dH5-His_POPC

26251

Nanodisc Assembly   Kit MSP1E3D1-His _DMPC

26253

Nanodisc Assembly   Kit MSP1E3D1-His _POPC

26311

Nanodisc   MSP1D1-His_DMPC (50 uL)

26313

Nanodisc   MSP1D1-His_POPC (50 uL)

26315

Nanodisc   MSP1D1-His_DMPG (50 uL)

26321

Nanodisc MSP1D1   dH5-His_DMPC (50 uL)

26323

Nanodisc MSP1D1   dH5-His_POPC (50 uL)

26351

Nanodisc MSP1E3D1-His_DMPC   (50 uL)

26353

Nanodisc   MSP1E3D1-His_POPC (50 uL)

26355

Nanodisc   MSP1E3D1-His_DMPG (50 uL)

26371

Nanodisc   MSP2N2-His_DMPC (50 uL)

26373

Nanodisc   MSP2N2-His_POPC (50 uL)

26421

Nanodisc MSP1D1 dH5-His_DMPC_Biotinyl_PE   (50 ul)

26711

Nanodisc mouse   MSP1D1-His_DMPC (50 uL)

26713

Nanodisc mouse   MSP1D1-His_POPC (50 uL)

26751

Nanodisc mouse   MSP1E3D1-His_DMPC (50 uL)

26753

Nanodisc mouse   MSP1E3D1-His_POPC (50 uL)

References:

1. Zeno, Wade F., et al. "Dynamics of crowding-induced mixing in phase separated lipid bilayers." The Journal of Physical Chemistry B 120.43 (2016): 11180-11190.

2. Efremov, Rouslan G., Christos Gatsogiannis, and Stefan Raunser. "Lipid nanodiscs as a tool for high-resolution structure determination of membrane proteins by single-particle cryo-EM." Methods in enzymology. Vol. 594. Academic Press, 2017. 1-30.

3. Carvalho, Vanessa, Joachim W. Pronk, and Andreas H. Engel. "Characterization of Membrane Proteins Using Cryo‐Electron Microscopy." Current protocols in protein science 94.1 (2018): e72.

4. Murugathas, Thanihaichelvan, et al. "Biosensing with Insect Odorant Receptor Nanodiscs and Carbon Nanotube Field-Effect Transistors." ACS applied materials & interfaces 11.9 (2019): 9530-9538.

5. Damiati, Samar, et al. "Albumin-bound nanodiscs as delivery vehicle candidates: Development and characterization." Biophysical chemistry 251 (2019): 106178.

6. Rues, Ralf-Bernhardt, et al. "Cell-free production of membrane proteins in Escherichia coli lysates for functional and structural studies." Heterologous Expression of Membrane Proteins. Humana Press, New York, NY, 2016. 1-21.

7. Gatsogiannis, Christos, et al. "Membrane insertion of a Tc toxin in near-atomic detail." Nature structural & molecular biology 23.10 (2016): 884.

8. Morozova, Kateryna, et al. "Structural and biological interaction of hsc-70 protein with phosphatidylserine in endosomal microautophagy." Journal of Biological Chemistry 291.35 (2016): 18096-18106.

9. Martens, Chloe et al. "Integrating hydrogen–deuterium exchange mass spectrometry with molecular dynamics simulations to probe lipid-modulated conformational changes in membrane proteins" Nature Protocols (2019): 18096-18106.

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